Hybridization-induced interfacial changes detected by non-Faradaic impedimetric measurements compared to Faradaic approach - Le Mans Université Accéder directement au contenu
Article Dans Une Revue Journal of Electroanalytical Chemistry Année : 2007

Hybridization-induced interfacial changes detected by non-Faradaic impedimetric measurements compared to Faradaic approach

Résumé

A biosensor for direct label-free DNA detection based on a polythiophene matrix is investigated by electrochemical impedance spectroscopy (EIS). Impedimetric experiments are performed with and without redox probe in solution. The non-Faradaic impedance measurements reveal two relaxation processes located at 50 Hz and 5 kHz, respectively. The first relaxation process, located at low frequencies, allows to detect biorecognition events by measuring the phase angle decrease, in accordance with a hindrance of the polaronic conduction. The second relaxation process, located at 5 kHz and originating from DNA modification, seems to increase with the length of the target sequence. These results suggest that this loaded support provides a platform for impedimetric detection of hybridization at high frequencies, leading to less time-consuming detection procedure. For a better understanding, results obtained in non-Faradaic mode are compared with Faradaic approach.

Dates et versions

hal-02458466 , version 1 (28-01-2020)

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Citer

Christelle Gautier, Charles Esnault, Charles Cougnon, Jean-François Pilard, Nathalie Casse, et al.. Hybridization-induced interfacial changes detected by non-Faradaic impedimetric measurements compared to Faradaic approach. Journal of Electroanalytical Chemistry, 2007, 610 (2), pp.227-233. ⟨10.1016/j.jelechem.2007.07.013⟩. ⟨hal-02458466⟩

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